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7-氨基放線菌素D (7-AAD)應(yīng)用：
7-Aminoactinomycin D (7-AAD), an analog of the antibiotic actinomycin D (sc-200906), is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase.

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制備儲(chǔ)備液 for7-Aminoactinomycin D

以下數(shù)據(jù)基于產(chǎn)品分子量 1270.45。 由于水合程度的不同，批次的特定分子量可能因批次而異，這將影響制備儲(chǔ)備溶液所需的溶劑體積。選擇批次從而根據(jù)批次分子量重新計(jì)算: 

濃度/溶劑體積/質(zhì)量	1 mg	5 mg	10 mg
0.02 mM	39.36 mL	196.78 mL	393.56 mL
0.1 mM	7.87 mL	39.36 mL	78.71 mL
0.2 mM	3.94 mL	19.68 mL	39.36 mL
1 mM	0.79 mL	3.94 mL	7.87 mL

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7-氨基放線菌素D (7-AAD)光譜

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7-氨基放線菌素D 實(shí)驗(yàn)注意事項(xiàng)：
1.實(shí)驗(yàn)前需戴好防護(hù)眼鏡，穿戴防護(hù)服和口罩，佩戴手套，避免與皮膚接觸。
2.實(shí)驗(yàn)過(guò)程中如遇到有毒或者刺激性物質(zhì)及有害物質(zhì)產(chǎn)生，必要時(shí)實(shí)驗(yàn)操作需要手套箱內(nèi)完成以免對(duì)實(shí)驗(yàn)人員造成傷害
3.實(shí)驗(yàn)后產(chǎn)生的廢棄物需分類存儲(chǔ)，并交于專業(yè)生物廢氣物處理公司處理，以免造成環(huán)境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

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