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偽胸苷

NMR下載 COA and HPLC MSDS下載
names：
Pseudothymidine
CAS號：
65358-15-8
MDL Number： MFCD04973713
MF(分子式)： C10H14N2O5 MW(分子量)： 242.23
EINECS:No data available Reaxys Number:No data available
Pubchem ID:375973985 Brand:BIOFOUNT

偽胸苷(65358-15-8,Pseudothymidine)是胸苷的C-核苷（C-nucleosid）類似物。

貨品編碼	規(guī)格	純度	價格 (￥)	現(xiàn)價(￥)	特價(￥)	庫存描述	數(shù)量	總計 (￥)
YZM000669-5mg	5mg	99.85%	￥ 6581.00	￥ 6581.00		2-3天	- +	￥ 0.00
YZM000669-1mg	1mg	99.85%	￥ 1852.50	￥ 1852.50		2-3天	- +	￥ 0.00

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中文別名	偽胸苷(65358-15-8);5-(2-脫氧-BETA-D-赤式-呋喃戊糖基)-1-甲基-2,4(1H,3H)-嘧啶二酮;
英文別名	Pseudothymidine(65358-15-8);5-Methyl-2'-Deoxypseudouridin;5-(2-Deoxy-beta-D-erythro-pentofuranosyl)-1-methyl-2,4(1H,3H)-pyrimidinedione;PSEUDOTHYMIDINE;5-Methyl-2'-Deoxypseudouridin;
CAS號	65358-15-8
Inchi	No data available
InchiKey	No data available
分子式 Formula	C10H14N2O5
分子量 Molecular Weight	242.23
溶解度Solubility	生物體外In Vitro:DMSO溶解度≥ 61.17 mg/mL(252.53 mM)*"≥" means soluble可溶, but saturation unknown溶解度未知.
性狀	固體粉末,Power
儲藏條件 Storage conditions	-20°C 3 years年 4°C 2 years年 / In solvent溶液中:-80°C 6 months月 -20°C 1 month月

偽胸苷(65358-15-8,Pseudothymidine)實驗注意事項：
1.實驗前需戴好防護眼鏡，穿戴防護服和口罩，佩戴手套，避免與皮膚接觸。
2.實驗過程中如遇到有毒或者刺激性物質及有害物質產生，必要時實驗操作需要手套箱內完成以免對實驗人員造成傷害
3.實驗后產生的廢棄物需分類存儲，并交于專業(yè)生物廢氣物處理公司處理，以免造成環(huán)境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

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產品說明	(65358-15-8,Pseudothymidine)偽胸苷是胸苷的C-核苷（C-nucleosid）類似物。
Introduction	(偽胸苷,65358-15-8)Pseudothymidine is a Cucleoside analog of thymidine.
Application1
Application2
Application3

偽胸苷(65358-15-8,Pseudothymidine)藥理學:

偽胸苷是胸苷的C-核苷（C-nucleosid）類似物。

警示圖
危險性	warning
危險性警示	Not available
安全聲明	H303吞入可能有害+H313皮膚接觸可能有害+H2413吸入可能對身體有害
安全防護	P264處理后徹底清洗+P280戴防護手套/穿防護服/戴防護眼罩/戴防護面具+P305如果進入眼睛+P351用水小心沖洗幾分鐘+P338取出隱形眼鏡（如果有）并且易于操作,繼續(xù)沖洗+P337如果眼睛刺激持續(xù)+P2393獲得醫(yī)療建議/護理
備注	實驗過程中防止吸入、食入，做好安全防護

S Lutz, et al. An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases. Nucleic Acids Res. 1999 Jul

Havemann SA, et al. Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure. Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):261-78.

偽胸苷(65358-15-8,Pseudothymidine)參考文獻：

1、Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure
Stephanie A Havemann , Shuichi Hoshika, Daniel Hutter, Steven A Benner

Abstract：Thermal denaturation and circular dichroism studies suggested that multiple (up to 12), sequential pseudothymidines, a representative C-glycoside, do not perturb the structure of a representative DNA duplex. Further, various Family A and B DNA polymerases were found to extend a primer by incorporating four sequential pseudothymidine triphosphates, and then continue the extension to generate full-length product. Detailed studies showed that Taq polymerase incorporated up to five sequential C-glycosides, but not more. These results constrain architectures for sequencing, quantitating, and analyzing DNA analogs that exploit C-glycosides, and define better the challenge of creating a synthetic biology using these with natural polymerases.

2、An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases
S Lutz , P Burgstaller, S A Benner

Abstract：DNA polymerases are desired that incorporate modified nucleotides into DNA with diminished pausing, premature termination and infidelity. Reported here is a simple in vitro assay to screen for DNA polymerases that accept modified nucleotides based on a set of primer extension reactions. In combination with the scintillation proximity assay (SPA[trade]), this allows rapid and simple screening of enzymes for their ability to elongate oligonucleotides in the presence of unnatural nucleotides. A proof of the concept is obtained using pseudo-thymidine (psiT), the C-nucleoside analog of thymidine, as the unnatural substrate. The conformational properties of psiT arising from the carbon-carbon bond between the sugar and the base make it an interesting probe for the importance of conformational restraints in the active site of polymerases during primer elongation. From a pool of commercially available thermostable polymerases, the assay identified Taq DNA polymerase as the most suitable enzyme for the PCR amplification of oligonucleotides containing psiT. Subsequent experiments analyzing PCR performance and fidelity of Taq DNA polymerase acting on psiT are presented. This is the first time that PCR has been performed with a C-nucleoside.

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